样品:(CYS)2 , LYS , ARG , HIS , ASP , GLU , GLN , ASN , GLY , SER , ALA , THR , PRO , TYR , VAL , PHE , MET ,
TRP , LEU+ILE;(半胱氨酸)、赖氨酸、地高辛、天冬氨酸、谷氨酸、谷氨酸、天冬氨酸、甘氨酸、丝氨酸、丙氨酸、苏氨酸、脯氨酸、酪氨酸、缬氨酸、苯丙氨酸、地中海、低浓铀+碘油
适用范围:Analysis of underivatized amino acids by LC/MS/MS;LC/MS/MS对未分解氨基酸的分析;
色谱柱:ULTRON AF-HILIC-CD (PLSS)
色谱柱型号:150 mm L × 2.0 mm I.D.
检测器:LC/MS/MS (ESI, Positive, MRM)
粒径:2 µm
流动相:A: 100 mM HCO2NH4(pH 3.0)/CH3CN = 10/90, B: 100 mM HCO2NH4(pH 3.0)/CH3CN = 70/30
流速:0.36 mL/min
洗脱梯度:20 minutes gradient, 88% - 60% with 10 minutes re-equilibration