样品预处理后使用Silica C18M 4E色谱柱分析黄曲霉毒素。
| (TFA method) | (PR method) | ||
|---|---|---|---|
| 1. | Derived Aflatoxin G1 | 1. | Aflatoxin G2 |
| 2. | Derived Aflatoxin B1 | 2. | Aflatoxin G1 |
| 3. | Aflatoxin G2 | 3. | Aflatoxin B2 |
| 4. | Aflatoxin B2 | 4. | Aflatoxin B1 |
(TFA method)
Column : Shodex Silica C18M 4E 4.6 mm I.D. x 250 mm)
Eluent : CH3CN/CH3OH/H2O=10/30/60
Flow rate : 1.0 mL/min
Detector : Fluorescence (Ex.365 nm, Em.450 nm)
Column temp. : 40 ℃
(PR method)
Column : Shodex Silica C18M 4E (4.6 mm I.D. x 250 mm)
Eluent : CH3OH/H2O=40/60
Flow rate : 0.7 mL/min
Detector : Fluorescence(Ex.365 nm, Em.450 nm)
Column temp. : 40 ℃