IEC DEAE-825 (弱阴离子交换色谱柱)分析人血清蛋白。
Sample : Human serum
Column : Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm)
Eluent : (A); 50 mM Tris-HCl buffer (pH8.0)
(B); (A) + 0.5 M NaCl
Linear gradient; 0 min to 60 min, (A) to (B)
Flow rate : 1.0 mL/min
Detector : UV (280 nm)
Column temp. : Room temp.