水溶性 SEC (GFC)色谱柱OHpak SB-805 HQ和SB-806 HQ分析人血清IgM。
Sample : 50 μLIgM, Immunoglobulin M from Human Serum(Wako Pure Chemical Industries, Ltd.) 0.1 %
Columns : Shodex OHpak SB-806 HQ + SB-805 HQ (8.0 mm I.D. x 300 mm each)
Eluent : 100 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate : 1.0 mL/min
Detector : UV (254 nm)
Column temp. : 30 ℃