RSpak DE-613 (反相色谱柱)分析二肽.。
Sample :
1. Gly-Val
2. Phe-Gly
3. Ala-Phe
4. Val-Phe
5. Tyr-Leu
Column : Shodex RSpak DE-613 (6.0 mm I.D. x 150 mm)
Eluent : (A); 0.05 % TFA in (H2O/CH3CN=96/4)
(B); 0.05 % TFA in (H2O/CH3CN=75/25)
Linear gradient: 0 to 30 min, (A) to (B)
Flow rate : 1.0 mL/min
Detector : UV (220 nm)
Column temp. : 30 ℃