Asahipak ES-502C 7C (弱阳离子交换色谱柱)分析四种标准蛋白质。
Sample :
1. Myoglobin
2. Ribonuclease A
3. Chymotrypsinogen A
4. Lysozyme
Column : Shodex Asahipak ES-502C 7C (7.5 mm I.D. x 100 mm)
Eluent : (A); 50 mM Sodium phosphate buffer (pH7.0)
(B); (A) + 0.5 M NaCl
Linear gradient; 0 min to 20 min, (A) to (B)
Flow rate : 1.0 mL/min
Detector : UV (280 nm)