下图为使用水溶性SEC(GFC)色谱柱PROTEIN KW-802.5和KW-803 对标准蛋白质的分离,KW-802.5适合分析分子量低于 150,000的蛋白质,KW-803孔径比KW-802.5大,适合分析分子量大于150,00
Sample : Molecular weight marker
1. Glutamate dehydrogenase
2. Lactate dehydrogenase
3. Enolase
4. Adenylate kinase
5. Cytochrome c
Columns : Shodex PROTEIN KW-802.5, KW-803 (8.0 mm I.D. x 300 mm each)
Eluent : 50 mM Phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate : 1.0 mL/min
Detector : UV (280 nm)
Column temp. : Room temp.