氨基酸 (3) (P-421S)

2023-07-11 11

色谱柱P-421S ；Alanine, Ala丙氨酸；Arginine, Arg精氨酸；Aspartic acid, Asp天冬氨酸；Cystine胱氨酸；Glutamic acid, Glu谷氨酸；Glycine, Gly甘氨酸；Histidine, His组氨酸；Isoleucine, Ile异亮氨酸；Leucine, Leu亮氨酸；Lysine, Lys赖氨酸；Methionine, Met蛋氨酸；Phenylalanine, Phe苯丙氨酸；

CXpak P-421S是氨基酸分析专用柱。以下是使用茚三酮显色法分析氨基酸的谱图。17种成分可以在50分钟内分析完毕。

Sample : 0.1 µM each,100 µL

1. Asp

2. Thr

3. Ser

4. Glu

5. Pro

6. Gly

7. Ala

8. Cystine

9. Val

10. Met

11. Ile

12. Leu

13. Tyr

14. Phe

15. Lys

16. NH3

17. His

18. Arg

Column       : Shodex CXpak P-421S (4.6 mm I.D. x 150 mm)
Eluent       : MCI BUFFER™ PH Kit (Mitsubishi Chemical Corporation)
               Low pressure step gradient; 
               PH‐1 (0 min), PH‐2 (0.2 min), PH‐3 (13.5 min), PH‐4 (23.2 min), 
               PH‐RG (47.0 min)
Reagent      : Ninhydrin Coloring Solution Kit for HITACHI
               (FUJIFILM Wako Pure Chemical Corporation)
               R1:R2=50:50
Flow rate    : (Eluent) 0.5 mL/min
               (Reagent) 0.35 mL/min
Detector     : VIS (570 nm)
Column temp. : 63 ℃
Reaction temp. : 120 ℃

网址信息：氨基酸 (3) (P-421S)|Shodex HPLC色谱柱和标准品

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