使用Asahipak ODP-50 6D(反相色谱柱)分析多肽。
Sample :
1. Val-Glu-Glu-Ala-Glu
2. Gly-Gly-Phe
3. Met-Enkephalin
4. Bradykinin
5. Angiotensin II
6. Angiotensin III
7. Phe-Phe-Phe
Column : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent : (1): (A); 0.1 M NaClO4 + 0.1 % Sodium phosphate (pH2)
(B); CH3CN
Linear gradient: 0 min to 60 min, (A)/(B)=90/10 to 60/40
(2): (A); 0.1 M NaClO4 + 0. 1% Sodium phosphate (pH9)
(B); CH3CN
Linear gradient: 0 min to 60 min, (A)/(B)=90/10 to 60/40
Flow rate : 1.0 mL/min
Detector : UV (220 nm)
Column temp. : 30 ℃