多肽 (1)

2023-07-10 5

色谱柱ODP-50 6D ;Angiotensin 血管紧张素;Bradykinin缓激肽;Gly-Gly-Phe甘氨酸-甘氨酸-苯丙氨酸;Met-Enkephalin脑啡肽;Phe-Phe-Phe苯丙氨酸;Val-Glu-Glu-Ala-Glu缬氨酸-谷氨酸-谷氨酸-丙氨酸-谷氨酸;

使用Asahipak ODP-50 6D(反相色谱柱)分析多肽。
  Sample :
1. Val-Glu-Glu-Ala-Glu
2. Gly-Gly-Phe
3. Met-Enkephalin
4. Bradykinin
5. Angiotensin II
6. Angiotensin III
7. Phe-Phe-Phe  
Column : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm) Eluent : (1): (A); 0.1 M NaClO4 + 0.1 % Sodium phosphate (pH2) (B); CH3CN Linear gradient: 0 min to 60 min, (A)/(B)=90/10 to 60/40 (2): (A); 0.1 M NaClO4 + 0. 1% Sodium phosphate (pH9) (B); CH3CN Linear gradient: 0 min to 60 min, (A)/(B)=90/10 to 60/40 Flow rate : 1.0 mL/min Detector : UV (220 nm) Column temp. : 30 ℃
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