使用 Asahipak ES-502N 7C (弱阴离子交换色谱柱)分析胶原和BSA 。
Sample :
1. Collagen(Pepsin-digested product of collagensoluble in acid extracted from pig skin)
2. BSA
Column : Shodex Asahipak ES-502N 7C (7.5 mm I.D. x 100 mm)
Eluent : 20 mM Monoethanolamine buffer (pH9.5) + 500 mM NaCl
Flow rate : 1.0 mL/min
Detector : UV (280 nm)
Column temp. : 30 ℃Courtesy of Dr. Shirai, Tokyo University of Agriculture and Technology