阿片肽 (1) (柱后衍生法)

2023-07-10 13

色谱柱ODP-50 6D ；Endorphin内啡肽;Leu-Enkephalin亮氨酸脑啡肽;Leu-Enkephalin-Arg亮氨酸精氨酸;Leu-Enkephalin-NH2亮氨酸-NH2;Met-Enkephalin脑啡肽;Met-Enkephalin-Arg精氨酸;Met-Enkephalin-Arg-Gly-Leu精氨酸-甘氨酸-亮氨酸;Met-Enkephalin-Arg-Phe精氨酸-苯丙氨酸;Met-Enkephalin-NH2甲基-脑啡肽-NH2;Met-Enkephalin-Thr-Ser-Glu-Lys丝氨酸-谷氨酸-赖氨酸;Tyr-Gly甘氨酸;Tyr-Gly-Gly酪氨酸甘氨酸;

使用Asahipak ODP-50 6D (反相色谱柱)通过柱后衍生法分析阿片肽。

Sample :

1. Tyr-Gly

2. Tyr-Gly-Gly

3. Tyr-Gly-Gly-Phe

4. Tyr-Phe

5. ME-Thr-ser-Glu-Lys

6. ME, Met-Enkephalin

7. LE, Leu-Enkephalin

8. ME-Arg, Met-Enkephalin-Arg

9. α-EDP, α-Endorphin

10. LE-Arg, Leu-Enkephalin-Arg

11. ME-NH2, Met-Enkephalin-NH2

12. γ-EDP, α-EDP-Leu

13. ME-Arg-Glu-Leu

14. LE-NH2, Leu-Enkephalin-NH2

15. ME-Arg-Phe

16. DNP 1-8

Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : (A); 50 mM Sodium borate (pH10.0)/H2O/CH3CN=4/15/1
               (B); 50 mM Sodium borate (pH10.0)/H2O/CH3CN=1/1/3
               Gradient; (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : Fluorescnce (Ex. 330 nm, Em. 430 nm)
Column temp. : Room temp.

Guo-Qing Zhang et al., Analytical Science, 6 (1990) 671.

网址信息：阿片肽 (1) (柱后衍生法)|Shodex HPLC色谱柱和标准品

本文导读