IEC QA-825 (强阴离子交换色谱柱) 与 IEC DEAE-825 (弱阴离子交换色谱柱)分析大豆脂肪氧化酶。样品的活性酶使用过氧 化氢来测量。实验发现箭头位置为活性酶。
Sample : Lipoxidase from soybean
Columns : (Left) Shodex IEC QA-825
(Right) Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm each)
Eluent : (Left) (A); 20 mM Ethanolamine-HCl buffer (pH9.0)
(Right) (A); 20 mM Tris-HCl buffer (pH8.0)
(B); (A) + 0.5 M NaCl
Linear gradient; 0 min to 60 min, (A) to (B)
Flow rate : 1.0 mL/min
Detector : UV (280 nm)
Column temp. : Room temp.