阿片肽 (2) (柱前衍生法)

2023-07-10 18

色谱柱ODP-50 6D ；Endorphin内啡肽；Leu-Enkephalin亮氨酸脑啡肽；Leu-Enkephalin-Arg亮氨酸精氨酸；Leu-Enkephalin-NH2亮氨酸-NH2；Met-Enkephalin脑啡肽；Met-Enkephalin-Arg-Gly-Leu精氨酸-甘氨酸-亮氨酸；Met-Enkephalin-Arg-Phe精氨酸-苯丙氨酸；Neoendorphin新内啡肽；Tyr-Gly-Gly-Phe酪氨酸-甘氨酸-苯丙氨酸；

Asahipak ODP-50 6D柱前衍生法分析阿片肽。

Sample :

1. Tyr-Gly-Gly-Phe

2. ME, Met-Enkephalin

3. LE, Leu-Enkephalin

4. LE-Arg

5. α-EDP, α-Endorphin

6. LE-NH2

7. DNP 1-7

8. [D-Ala2] LE-NH2

9. ME-Arg-Gly-Leu

10.γ-EDP

11. ME-Arg-Phe

12. DNP 1-8

13. β-NED,β-Neoendorphin

14. α-NED,β-NED-Lys

15. DNP 1-9

16. DNP 1-10

17. DNP-B

Column       : Shodex Asahipak ODP-50 6D (6.0mmI.D. x 150mm)
Eluent       : (A); 0.1M Borate buffer(pH9.5)/H2O/CH3CN=10/39/1
               (B); 0.1M Borate buffer(pH9.5)/H2O/CH3CN=2/3/5
               Gradient:(A) to (B)
Flow rate    : (0 to 25min); 0.8mL/min, (25 to 55min); 1.0mL/min,
               (after 55min); 0.8mL/min 
Detector     : Fluorescence(Ex. 330nm, Em. 430nm)
Column temp. : Room temp.

Guo-Qing Zhang et al., Chem. Pharm. Bull., 39 (1991) 126.

网址信息：阿片肽 (2) (柱前衍生法)|Shodex HPLC色谱柱和标准品

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