使用Asahipak ES-502C 7C (弱阳离子交换色谱柱)分析肌球蛋白亚片断。
Sample : Myosin subfragment
Column : Shodex Asahipak ES-502C 7C (7.5 mm I.D. x 100 mm)
Eluent : (A); 5 mM Sodium phosphate buffer (pH7.0)
(B); (A) + 0.1 M NaCl
Linear gradient; 0 min to 25 min, (A) to (B)
Flow rate : 1.0 mL/min
Detector : UV (220 nm)
Column temp. : 10 ℃Courtesy of Dr. Muno, Juntendo University