IEC CM-825 (弱阳离子交换色谱柱)分析马胰核糖核酸酶。这种酶含有相当少量的杂质。
Sample: 0.5 % Ribonuclease A (pI=9.3), 30 μL
Column : Shodex IEC CM-825 (8.0 mm I.D. x 75 mm)
Eluent : (A); 20 mM Sodium phosphate buffer (pH7.0)
(B); (A) + 0.5 M NaCl
Linear gradient; 0 min to 60 min, (A) to (B)
Flow rate : 1.0 mL/min
Detector : UV (280 nm)
Column temp. : 25 ℃