使用聚合物基质HILIC色谱柱HILICpak VN-50 4D配合蒸发光散射检测器(ELSD)分析低聚木糖,可以分离至13聚体。
Sample : 10 μLXylooligosaccharides 5 mg/mL (in 50 % CH3CN)
1. Xylose
2. Xylobiose
3. Xylotriose
4. Xylotetraose
5. Xylopentaose
7. Xylohexaose
8. Xyloheptaose
9. Xylooctaose
10. Xylodecaose
11. Xyloundecaose
12. Xylododecaose
13. Xylotridecaose
Column : Shodex HILICpak VN-50 4D (4.6 mm I.D. x 150 mm)
Eluent : (A); 0.1 % NH3 aq. (B); CH3CN
Linear gradient; (B %) 80 % to 50 % (0 to 20 min)
Flow rate : 1.0 mL/min
Detector : ELSD
Column temp. : 40 ℃