N-乙酰壳寡糖 (2) (VN-50 4D)

2023-07-14 1

色谱柱HILICpak VN-50 4D； N-Acetyl-D-glucosamineN-乙酰-D-葡糖胺；N-Acetylchitooligosaccharides, Chitin oligosaccharidesN-乙酰壳寡糖、壳寡糖；di-N-Acetylchitobiose二-N-乙酰壳二糖；hepta-N-Acetylchitohexaose七-N-乙酰壳六糖；hexa-N-Acetylchitohexaose六-N-乙酰壳六糖；penta-N-Acetylchitopentaose五-N-乙酰几丁质酶；tetra-N-Acetylchitotetraose四-N-乙酰壳四糖；tri-N-Acetylchitotriose三-N-乙酰壳三糖；

使用聚合物基质HILIC色谱柱HILICpak VN-50 4D分析配合蒸发光散射检测器(ELSD)分析N-乙酰壳寡糖。可以分离至7聚体的样品。

Sample : 10 μL

N-Acetylchitooligosaccharides 5mg/mL (in 50 % CH3CN)

1. N-Acetyl-D-glucosamine

2. di-N-Acetylchitobiose

3. tri-N-Acetylchitotriose

4. tetra-N-Acetylchitotetraose

5. penta-N-Acetylchitopentaose

6. hexa-N-Acetylchitohexaose

7. hepta-N-Acetylchitohexaose

Column       : Shodex HILICpak VN-50 4D (4.6 mm I.D. x 150 mm)
Eluent       : (A); H2O (B); CH3CN
               Linear gradient; (B %) 80 % to 65 % (0 to 10 min)
Flow rate    : 1.0 mL/min
Detector     : ELSD
Column temp. : 60 ℃

色谱柱：N-乙酰壳寡糖 (2) (VN-50 4D)|Shodex HPLC色谱柱和标准品

本文导读