由于电雾式检测器 (CAD)检测从色谱柱洗脱出来的任何待测成分,因此对基线影响大。而聚合物氨基柱 Asahipak NH2P-50 4E填料流失很少,因此相比较于硅胶氨基柱,基线稳定噪音小。
Sample : 40 μg/mL each, 5 μL
1. Fructose
2. Glucose
Columns : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Silica based amino column from other manufacturer (4.6 mm I.D. x 250 mm)
Silica based amide column from other manufacturer (4.6 mm I.D. x 150 mm)
Eluent : H2O/CH3CN=20/80
Flow rate : 1.0 mL/min
Detector : Corona charged aerosol
Column temp. : 30 ℃ (Amino column), 80 ℃ (Amide column)