使用有机酸分析用色谱柱RSpak KC-811分析有机酸。流动相中加入环糊精,可以缩短丁酸、戊酸等长碳链的有机酸的洗脱时间。
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Sample : 5 mM each, 20 μL
1. Fumaric acid
2. Succinic acid
3. Lactic acid
4. Formic acid
5. Acetic acid
6. Propionic acid
7. n-Butyric acid
8. Isobutyric acid
9. n-Valeric acid
10. Isovaleric acid
Columns : Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
Eluent : 2 mM HClO4 + 4 mM α-cyclodextrin (pH3.0)
Flow rate : (Eluent); 1.0 mL/min, (Reagent); 0.5 mL/min
Detector : VIS (430 nm) (Post column method)
Column temp. : 47 ℃