使用阳离子交换色谱柱Asahipak ES-502C 7C分析尿液中的儿茶酚胺。
Column : Shodex Asahipak ES-502C 7C (7.5 mm I.D. x 100 mm)
Eluent : Sodium succinate-borate-ethylenediaminetetraacetate
0.05 mol/L-0.015 mol/L-0.05 mmol/L, pH6.7
Reaction reagent : (A); 0.1 M glycylglycine containing 0.1 M tartaric acid +
0.1 M boric acid + 2 mM zinc sulphate (pH6.5)
(B); 0.25 M potassium borate buffer (pH8.4)
containing 0.01 %(w/v) hexacyanoferrate(III)
Flow rate : (Eluent) 1.0 mL/min, (Reaction reagent) 0.35 mL/min each
Detector : Fruorescence (Ex 350 nm, Em 500 nm)
Column temp. : 30 ℃
Reaction temp. : 90 ℃