色谱柱DEAE-825；Adenosine diphosphate, ADP二磷酸腺苷；Adenosine triphosphate, ATP三磷酸腺苷；Adenylic acid, Adenosine monophosphate, AMP腺苷酸、一磷酸腺苷；Cytidine 5'-diphosphate, 5'-CDP5'-二磷酸胞苷；Cytidine triphosphate, CTP三磷酸胞苷；Cytidylic acid, Cytidine monophosphate, CMP胞苷酸、一磷酸胞苷；Guanosine diphosphate, GDP二磷酸鸟苷；Guanosine triphosphate, GTP三磷酸鸟苷；Guanylic acid, Guanosine monophosphate, GMP鸟苷酸、单磷酸鸟苷；Uridine diphosphate, UDP尿苷二磷酸；Uridine triphosphate,UTP尿苷三磷酸；Uridylic acid, Uridine monophosphate, UMP尿苷酸、一磷酸尿苷、尿苷；
IEC DEAE-825 (弱阴离子交换色谱柱)分析核苷酸。
Sample :
1. CMP
2. AMP
3. UMP
4. GMP
5. CDP
6. ADP
7. UDP
8. CTP
9. GDP
10. ATP
11. UTP
12. GTP
Column : Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm)
Eluent : (A); 0.1 M NaH2PO4 aq./CH3CN=80/20
(B); 0.5 M NaH2PO4 aq./CH3CN=80/20
Linear gradient; 0 min to 60 min, (A) to (B)
Flow rate : 1.0 mL/min
Detector : UV (260 nm)
Column temp. : 25 ℃
