使用三根不同的色谱柱RSpak DM-614, NN-814和DE-613来分析鸟嘌呤和色氨酸。
Columns : (left); Shodex RSpak DM-G 4A (4.6 mm I.D. x 10 mm)
+ DM-614 (6.0 mmI.D. x 150 mm)
(Center); Shodex RSpak NN-G (6.0 mm I.D. x 50 mm)
+ NN-814 (8.0 mm I.D. x 250 mm)
(Right); Shodex RSpak DE-G 4A (4.6 mmI.D. x 10 mm)
+ DE-613 (6.0 mm I.D. x 150 mm)
Eluent : (Left); 0.3 M K2HPO4 (pH8.6)
(Center); 0.1 M NaH2PO4 (pH3.0 adjusted by H3PO4)
(Right); 0.23 M K2HPO4 aq./CH3OH=80/20
Flow rate : (Left); 0.8 mL/min, (Center); 1.0 mL/min, (Right); 0.8 mL/min
Detector : (Left); UV (278 nm), (Center); RI, (Right); UV (278 nm)
Column temp.: (Left); 30 ℃, (Center); 50 ℃, (Right); 30 ℃