转移核糖核酸 (1) (DEAE-825)

2023-07-12 108

色谱柱IEC DEAE-825；Ribonucleic acid, RNA核糖核酸;Transfer RNA, tRNA转移核糖核酸;

IEC DEAE-825(弱阴离子交换色谱柱)分析转移核糖核酸。

  Sample : 10 µLtRNA (from E.Coli) 0.4 %  

Column : Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm) Eluent : (A); 20 mM Sodium phosphate buffer (pH6.6) + 250 mM KCl + 5 M Urea + 0.5 mM EDTA (B); (A) + 250 mM KCl Linear gradient: 0 min to 150 min, (A) to (B) Flow rate : 1.0 mL/min Detector : UV (260 nm) Column temp. : Room temp.

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